The Splenocyte-Conditioned Media Inhibit Breast Cancer MCF-7 Cell Growth, Associated with Increased Th2/Th1 Cytokine Secretion Ratio in the Media using Five Selected Polysaccharides
The Splenocyte-Conditioned Media Inhibit Breast Cancer MCF-7 Cell Growth, Associated with Increased Th2/Th1 Cytokine Secretion Ratio in the Media using Five Selected Polysaccharides
Journal of Immunobiology (Hilaris Publishing S.L) has announced almost 50% discount on article processing charge to commemorate its 10th Anniversary.
Journal of Immunobiology is an open access, peer-reviewed journal that focuses and welcomes submissions on all aspects of Immunobiology, Immunology, Autoimmunity, Innate Immunity, Adaptive Immunity, immunization, Immunogenetics, Immune Disorders, Immunodeficiency, Immune inflammation, Immunoinformatics, Clinical Immunology, Allergy & Immunology, Clinical & Vaccine Immunology, Inflammatory Disorders, Autoimmune Disorders, Immunotherapy and Immunopathology of infectious, allergic and autoimmune disease etc.
It gives us great pleasure to announce the call for paper on the occasion of 10th Anniversary of the Journal at special and hefty discount of up to 50 % on one-time article processing charge. Prospective academicians and scientists are encouraged to utilize this opportunity to get their articles reviewed, processed and published at relatively faster pace and at lower charges. In addition to this, the authors who publish with us during the year-long celebrations will also be eligible for academic awards recommended by the editorial panel.
The Archive page contains wide variety of articles such as Research / Review / Case reports / short communication / Mini review / Prospective / Letter to Editors Etc. We would like introduce a Research article which has been spread to the widest audience of experts; and thus increased in readership, citations and altimetry score.
Abstract: To unravel the cause and possible prevention of breast cancer, five potential polysaccharides from guava seed (GSPS), common buckwheat (CBPS), bitter buckwheat (BBPS), red Formosa lambsquarters (RFLPS) and yellow Formosa lambsquarters (YFLPS) were selected to treat human breast cancer MCF-7 cells with direct action or indirect tumor immunotherapy using polysaccharide-treated splenocyte-conditioned media (SCM). Five different polysaccharides were partially characterized. Cytokine levels produced by type 1 helper T-cells [Th1; interleukin (IL)-2 and interferon-γ] and type 2 helper T-cells (Th2; IL-4, IL-5 and IL-10) in SCM were determined using ELISA. Breast cancer MCF-7 cell viability was determined using an MTT assay. The association between cytokine levels in SCM and the corresponding breast cancer MCF-7 cell viability was investigated using the Pearson product-moment correlation coefficient. Total carbohydrate and protein contents indicated that these isolated polysaccharides might be proteopolysaccharides or glycoproteins. Molecular weights of purified polysaccharides fractions from the five isolated polysaccharides were determined. All 5 selected crude polysaccharides, in particular GSPS, BBPS and RFLPS, significantly increased IL-10/IL-2 cytokine secretion ratios by splenocytes. Treatment with SCM prepared in the presence or absence of the polysaccharides, but not the direct action of the polysaccharides, significantly inhibited MCF-7 cell viability. GSPS-, BBPS- and RFLPS-treated SCM demonstrated slightly enhanced inhibitory effects against breast cancer MCF-7 cell viability. There were negative correlations between MCF-7 cell viabilities and IL-10 cytokine secretion levels, as well as IL-10/IL-2 cytokine secretion ratios in the corresponding SCM. The present findings suggested that an increased Th2/Th1 cytokine secretion ratio, particularly an increase in IL-10, by immune cells including T lymphocytes, may inhibit MCF-7 cell growth in the tumor microenvironment. GSPS, BBPS and RFLPS may be further applied to treat human breast cancer via tumor immunotherapy by increasing the Th2/ Th1 cytokine secretion ratio.
To have a glance at the full length manuscript, you can visit us at our archive page and Currently, Journal’s Archive is holding not only normal issues but also focusing on special issues. The purpose of special issues is to publish the most exciting research with respect to the subjects of Immunology / Immunobiology and to provide a rapid turn-around time regarding reviewing and publishing, and to publicize the articles freely for research, teaching and reference purposes. Submit manuscript of your research articles or special issue articles online through manuscript submission or forwarded to the Editorial Office at immunobio@immunologyjournals.org
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