Genomic Analysis of Bacillus megaterium


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The rhizobacterium Bacillus megaterium is well known for encouraging plant development, but the precise chemical pathways involved are yet unknown. The purpose of this study was to clarify the effects of B. megaterium HT517 on greenhouse tomato growth and development, disease control, and its mechanism of action. To test the impact of B. megaterium on tomato growth, a pot experiment was carried out using the HT517 group (cfu/pot) and the control group (inoculated with the same amount of sterilised suspension).  To investigate the antagonistic effect of B. megaterium and Fusarium oxysporum f.sp. lycopersici, two experiments—a plate confrontation experiment and an antagonistic experiment—were undertaken.The chemical profile and metabolic route of HT517 were studied using liquid chromatography-mass spectrometry.

Utilizing PacBio+Illumina HiSeq sequencing, the maps of the samples were sequenced. The former Soviet researcher Monkina discovered Bacillus megaterium var. phosphaticum (B. megaterium) in 1935 and used it to breakdown organophosphorus chemicals in soil. He also began research on the artificial synthesis of phosphorus bacterial fertiliser. B. megaterium, a typical microbial fertiliser strain that plays an extraordinary role in dissolving phosphate and potassium, boosting development, and preventing plant diseases, has been the subject of extensive research in recent years due to the widespread use of microbial fertilisers in agriculture. The availability of phosphorus in the soil was poor, primarily in the form of insoluble inorganic or organophosphorus, which was challenging for plants to directly absorb and use, despite the soil's high absolute phosphorus level. Inorganic phosphorus is currently thought to dissolve mainly through the direct dissolution of insoluble phosphate through the secretion of organic acids (such as lactic acid, succinic acid, gluconic acid, citric acid, and oxalic acid) and the release of phosphate through the chelation of metal ions in soil. Enzymatic hydrolysis is the main mechanism of organophosphorus dissolution. Additional research revealed that B. megaterium JD-2 had a potent ability to dissolve both organic and inorganic phosphorus, and that after being infused into soil, its fermentation broth could raise the accessible phosphorus level by two times. When a microbial inoculant containing B. megaterium and Paenibacillus mucilaginosus was applied to weakly salinized soil planted with tomato, watermelon, and melon, the soil's accessible P concentration increased noticeably by 22.8%, 135%, and 95.1%.